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The Center for the Integrated Modeling and Analysis of the Gulf Ecosystem

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Online Monitoring of Crude Oil Biodegradation at Elevated Pressures

  • Thursday, 16 October 2014 17:40

Online Monitoring of Crude Oil Biodegradation at Elevated Pressures

Abstract

In order to study the biodegradation of crude oil spilled in the deep sea, incubations of deep-sea-bed sediments and crude oil were carried out in a high-pressure reactor, but monitoring the biodegradation of oil at high pressure is limited by sampling because the volatile crude oil components are partly lost during depressurization. Moreover, the seawater-oil-sediments multiphase system cannot be sampled representatively. The aerobic oil biodegradation can also be monitored indirectly by measuring the oxygen consumed and the carbon dioxide produced. In this paper, the O2 and CO2 concentrations were monitored in a reactor with transparent windows using chemical-optical sensors. To compare the effect of pressure on the biodegradation of oil, two pressure regimes were compared: atmospheric pressure (1 bar) and 150 bar, corresponding to 1500mdepth of the Deepwater Horizon’s well at the Gulf of Mexico. Only in the experiments where deep-sea sediments were added, the oxygen concentration decreased while the carbon dioxide and the bacterial concentration increased. In experiments where no sediment was added, the values for the oxygen and carbon dioxide remained constant. This proved that deep-sea sediments contained microorganisms, which could degrade crude oil at both 1 and 150 bar. To our knowledge, this is the first time where O2 and CO2 were monitored online during crude oil biodegradation at high pressure in the laboratory.

Ref: Valladares, A. G., Kadimesetty, S., Achatz, A., Schedler, M., Muller, R. Online Monitoring of Crude Oil Biodegradation at Elevated Pressures, IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing, 2014, 99(PP), 1-7. (in press)

Note: This publication is not open access.  Please contact the authors for more information.

A. G. Valladares Ju´arez, H. S. Kadimesetty, M. Schedler, and R. M¨uller are with the Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg 21073, Germany (e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.).

D. E. Achatz is with the PreSens Precision Sensing GmbH, Regensburg 93053, Germany, and also with the Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany (e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.).