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Kimberly Pause
Education: 2003 B.S. Biological Sciences, Florida State University 2007 PhD. Biomedical Sciences – Genetics Concentration, University of Florida
Project: Isolation and characterization of viruses from an acid mine drainage system Acid mine drainage (AMD) is a worldwide environmental problem associated with past mining activity. Within disused mines, extreme environmental conditions may develop that are characterized by high levels of dissolved sulfur, iron, and other metals as well as very low pH (0.5-1.0). While unique communities of bacteria and archaea have been identified in these systems, viruses and interactions with their hosts have yet to be fully characterized. We are working in collaboration with the Banfield lab at the University of California at Berkeley (UCB), who have been studying this system for many years. Methods for viral isolation from the AMD biofilm are being optimized for each sampling location, and viral concentrates are being generated for metagenomic sequencing and proteomics studies in collaboration with Oak Ridge National Laboratory (ORNL) and UCB. Viral genomes will be reconstructed from metagenomic sequences, allowing for estimates of viral community structure. The level of viral diversity will be assessed and compared to bacterial and archaeal diversity in the AMD system in collaboration with UCB. Viral morphology will be characterized using TEM and cryo-electron tomography in collaboration with Lawrence Berkeley National Laboratory (LBNL). This project is funded by a Genomes to Life grant from the Department of Energy. Online CV: http://myprofile.cos.com/kpause Publications: Dissertation Pause K. C. Conservation genetics of the Florida manatee, Trichechus manatus latirostris, University of Florida Doctoral Dissertations, December 2007 Peer-Reviewed Publications Pause K. C., M. E. Kellogg, A. M. Clark, C. A. Beck, M. K. Oli, R. K. Bonde, P. M. McGuire. (in prep). A survey of the genetic diversity and population structure of Florida manatees (Trichechus manatus latirostris). Pause K. C., C. Nourisson, A. M. Clark, M. E. Kellogg, R. K. Bonde, P. M. McGuire. (2007). Polymorphic microsatellite DNA markers for the Florida manatee (Trichechus manatus latirostris). Molecular Ecology Notes. 7, 1073-1076. [pdf] Pause K. C., R. K. Bonde, P. M. McGuire, R. T. Zori, B. A. Gray (2006) G-banded karyotype and ideogram for the North Atlantic right whale (Eubalaena glacialis). Journal of Heredity. 97, 303-306 [pdf]
Presentations: Oral Presentations Pause K. C. (April 2008) Conservation genetics of Florida manatees, Sirenian Population Genetics Workshop, Orlando, FL Pause K. C., A. M. Clark, R. K. Bonde, P. M. McGuire (April 2006) A preliminary analysis of gene flow and population structure of the Florida manatee, Wildlife Society of Florida Spring Meeting, Melbourne, FL Pause K. C., A. M. Clark, R. K. Bonde, P. M. McGuire (December 2005) Genetic Studies of the Florida Manatee, Sirenian International Symposium, San Deigo, CA For poster presentations, please see my online CV.
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