Marine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South FloridaMarine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida

RESEARCH - SENSOR RESEARCH

Summary
There is a need for the development of autonomous platforms for monitoring a wide range of chemical and biological targets in the oceans. Such platforms should be able to telemeter information to shore-based monitoring stations in near real time. The goal of our work is to develop an Autonomous Microbial Genosensor (AMG) to detect specific microbial targets in coastal or oceanic waters. Our instrumentation is based upon Nucleic Acid Sequence Based Amplification (NASBA) of microbial RNA targets and fluorescence detection using molecular beacons. We have successfully developed hand-held instruments for microbial target detection using this technology and we are now employing it to the greater challenge of an autonomous sensor. Our initial target is the Florida Red tide organism, Karenia brevis. Our current prototype collects water samples, filters cells and extracts RNA, and performs amplification autonomously. Our prospective second generation AMG will incorporate microfluidic liquid processing, array technology, and intensified light detection to improve the design and function of platforms capable of detecting a range of target organisms.
Center for Ocean Technology
National Science FoundationUSF College of Marine Science
Office of Naval Research

Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida Copyright © 2008, University of South Florida, College of Marine Science 140 7th Avenue S., St. Petersburg, FL 33701 Marine Microbiology Group - College of Marine Science - University of South Florida
Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida Marine Microbiology Group - College of Marine Science - University of South Florida