Natalie Sawaya is a PhD candidate in Dr. Mya Breitbart’s Marine Genomics Lab at the College of Marine Science in the University of South Florida. Natalie’s research focuses on using molecular techniques to study the diversity marine organisms.
Natalie’s current project focuses on studying gokushoviruses in the Red Sea and the Florida Keys. Gokushoviruses are single-stranded DNA (ssDNA) viruses that infect bacteria and are ubiquitous in marine environments, but little is known about their diversity and abundance. This knowledge gap is partly due to the methodological limitations associated with studying ssDNA phages. In order to elucidate Gokishoviruses ecological role Natalie will be looking at their diversity using next generation sequencing and will be quantifying their abundance using a new molecular technique known as the polony method in collaboration with the Lindell Lab.
Natalie has also worked as a part of the Marine Biodiversity Observation Network (MBON) project which aims to assess the diversity of marine organisms at various marine sanctuaries through metabarcoding environmental DNA (eDNA). Environmental DNA is the DNA left behind by organisms in the water through shedding of cells, scales, mucus and fecal matter. Analyzing eDNA enables a water sample to provide the composition of an entire ecosystem at a higher resolution than visual surveys alone, which will be very useful to determining the health and understanding the dynamics of that ecosystem. Get to know Natalie Sawaya.
Contact Natalie: email@example.com
Photos by Amanda Sosnowski at Terrene Visions (www.terrenevisions.com @terrenevisions)
Christina is a second year Master’s student working with Dr. Kate Hubbard (FWC) and Dr. Mya Breitbart. She uses genetic analyses to identify and quantify phytoplankton diversity. Genetic techniques are used to differentiate phytoplankton species that are easily distinguished using more traditional techniques such as light microscopy. One of the projects she is currently working on includes using ARISA (Automated Ribosomal Intergenic Spacer Analysis) to identify species in the diatom genus Pseudo-nitzschia from samples collected in offshore, coastal, and estuarine waters.
Contact Christina: firstname.lastname@example.org
Caitlyn is in her first year as a M.S. student working with Dr. Kristen Buck and Dr. Mya Breitbart. Her research will explore the potential interaction between iron and marine viruses. Iron, a trace metal, is essential to life and plays a vital role in primary production, yet it is found in very small quantities in the open ocean. While a variety of factors, such as organic complexation, can lead to a decrease in bioavailable iron, Caitlyn’s research will investigate how marine viruses may be capturing iron, using it as a mechanism to invade host organisms (the Ferrojan Horse hypothesis), and effectively decreasing iron’s bioavailability in the open ocean. Get to know Caitlyn Parente.
Contact Caitlyn: email@example.com
Keith is a first year M.S. student in Dr. Breitbart’s marine genomics lab. His research involves investigating the spawning patterns of fishes in the Gulf of Mexico by DNA barcoding fish eggs collected from the West Florida Shelf.
Contact Keith: firstname.lastname@example.org